JAX Seminars

Kathy Krentz, Director of UW Animal Models Core: Welcome and Introduction Mouse Nomenclature

1:00 PM - 1:20 PM CT

Understanding mouse nomenclature enables you to select optimal mouse models and control strains for your research to generate the most reliable and reproducible data.

• Interpreting the nomenclature of inbred, mutant, and genetically modified mouse strains
• Considerations for selecting control mice

Essential Tips for Choosing and Using Mouse Models in Research

1:20 PM - 2:30 PM CT

Would you like to find mouse model information essential to conducting your research quickly? We will share strategies for navigating publicly available, information-rich databases and discuss how mouse strain choice impacts research data quality and reproducibility during this seminar. Join us to learn about:

• Easily finding mouse strains to support your research
• The research value of using proper strain nomenclature in your publications
• Selecting the appropriate mouse strain background
• Choosing optimal control mice
• Ensuring the genetic integrity of your mouse colony

The Consequences of Sex Bias in Preclinical Research

2:30 PM - 3:30 PM CT

For mouse-based research investigating new cures for human diseases, one sex is far too often selected over the other. This sometimes happens for sound scientific reasons but omitting sex as a biological variable has several consequences. Single sex studies can lead to skewed research, translatability issue, flawed conclusions, and raise concerns concerning ethical practices and the 3Rs. During this presentation, you will learn more about:

• Reasons why male or female mice are sometimes preferred
• The scientific impact sex bias has on multiple biological disciplines
• The significant effect of sex bias on animal welfare
• 3R guidelines and recommendations to eliminate sex bias in preclinical studies

How to Prevent Your Newly Trained MIce from Taking Over Your Laboratory

1:00 PM - 2:00 PM

Do you need a unique mouse model that does not exist or is not available? Fortunately, many cost effective options for generating new models are now available. However, determining which background strains, genetic modification techniques, and breeding strategies to use for developing your new model can be complicated. During this presentation, we will share experience-based insights to help you with decision making during the model development process. Join us as we discuss approaches and considerations for mouse model generation, including:

• Strategies to generate indel, knockout, and knockin mutations using CRISPR/Cas9, transgenics, and ES cells
• Choosing the optimal genetic background for your new mouse model
• Selecting founder mice, breeding strategies, and phenotyping
• Streamlining new model generation using The Jackson Laboratory’s expertise and services

Kathy Krentz, Director of UW Animal Models Core: Welcome and Using Cre-Lox System to enhance your research

9:30 AM - 10:30 AM CT

Cre-lox technology is a widely used and sophisticated tool to create general knockouts, conditional knockouts, and reporter strains. Researchers worldwide utilize these models for various applications, including specific disease research and visualization of mouse anatomy

• How Cre-lox overcomes the limitations of traditional knockout modeling technology
• Implementing Cre-lox technology to model various types of cancer, neurodegenerative disorders, and other human diseases

Introduction to In Vivo Platforms for Cancer Immunotherapy Research

10:30 AM - 11:30 AM CT

During this seminar, we will provide an overview of in vivo platforms being used to improve the translational relevance of cancer immunotherapies. Join us as we discuss:

• Current approaches for cancer immunotherapy
• Types of cancer immunotherapeutics that have been characterized and validated in mouse models
• Innovative humanized platforms for immuno-oncology drug development

Comparing Mouse Models for Obesity and Diabetes Research

12:30 PM - 1:30 PM CT

Would you like to learn about the strengths and weaknesses of popular mouse models of human type 2 diabetes and obesity? Join this seminar as we discuss:

• The benefits and limitations of some common mouse strains used to study metabolic disease
• How genetic background and sex influence metabolic phenotypes
• Environmental factors that can influence phenotype and tips to help promote reproducible results
• Preclinical efficacy study data using reference compounds to modify metabolic phenotypes

Generating Better Mouse Models for Your Research: Approaches and Considerations

1:00 PM - 2:00 PM

Do you need a unique mouse model that does not exist or is not available? Fortunately, many cost effective options for generating new models are now available. However, determining which background strains, genetic modification techniques, and breeding strategies to use for developing your new model can be complicated. During this presentation, we will share experience-based insights to help you with decision making during the model development process. Join us as we discuss approaches and considerations for mouse model generation, including:

• Strategies to generate indel, knockout, and knockin mutations using CRISPR/Cas9, transgenics, and ES cells
• Choosing the optimal genetic background for your new mouse model
• Selecting founder mice, breeding strategies, and phenotyping
• Streamlining new model generation using The Jackson Laboratory’s expertise and services